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PHYTOCHEMICAL, PROXIMATE AND IN-VITRO ANTIOXIDANT ANALYSES OF Ageratum conyzoides LEAF

Authors

  • A. T. Adeoye Department of Animal Health Technology, Federal College of Animal Health and Production Technology, Moor Plantation, Apata, Ibadan
  • S. A. Koleosho Department of Pasture and Range Management, Federal College of Animal Health and Production Technology, Moor Plantation, Apata, Ibadan
  • O. J. Okuneye Department of Veterinary Laboratory Technology, Federal College of Animal Health and Production Technology, Moor Plantation, Apata, Ibadan
  • E. O. Ogbaji Department of Animal Health Technology, Federal College of Animal Health and Production Technology, Moor Plantation, Apata, Ibadan
  • O. C. Nwufoh Department of Veterinary Laboratory Technology, Federal College of Animal Health and Production Technology, Moor Plantation, Apata, Ibadan

DOI:

https://doi.org/10.52417/ojas.v4i2.517

Abstract

Before the emergence of pharmaceuticals, different cultures worldwide depended on medicinal plants to combat diseases. Ageratum conyzoides, an annual herb that possesses a rich record of medicinal use has been recognized for its pharmacological properties. This study aimed to examine the proximate content analysis, phytochemical components plus in-vitro antioxidant potentials of ethanol extract of A.  conyzoides leaf (ELEAC). Standard procedures were employed to conduct proximate and mineral composition analysis as well as qualitative and quantitative phytochemical assessments, while in vitro antioxidant assays were done using 1,1- Diphenyl2-picryl hydrazyl (DPPH) radical scavenging, Ferric Reducing Antioxidant Power (FRAP), Total Phenol Content (TPC) assays. Qualitative phytochemical assessment recorded the presence of saponins, tannins, terpenoids, alkaloids, phenols, and flavonoids. Proximate and mineral analysis revealed high carbohydrate content (84.97%), along with crude protein (1.53%), crude fat (3.27%), moisture (9.00%), Magnesium (42.30 mg/L), Sodium (73.21 mg/L) and Iron (4.30 mg/L). The extract exhibited a % DPPH radical scavenging activity of 44.63 at 200 µg/ml and 56.05 at 1000 µg/mL while the FRAP assay indicated a concentration-dependent increase in absorbance from 0.544 at 20 µg/mL to 0.756 at 100 µg/ml. TPC of the extract was quantified as 1.40 µg gallic acid equivalent/ mg of residue at 200 µg/mL and 2.22 µg gallic acid equivalent/ mg of residue at 1000 µg/mL. In conclusion, this study affirms the presence of medicinal phytochemicals in A. conyzoides. The identified phytochemicals, nutrients, and minerals with the demonstrated antioxidant activities, lend credibility to the traditional use of A, conyzoides in herbal medicine.

Published

2023-12-31

How to Cite

Adeoye, A. T., Koleosho, S. A., Okuneye, O. J., Ogbaji, E. O., & Nwufoh, O. C. (2023). PHYTOCHEMICAL, PROXIMATE AND IN-VITRO ANTIOXIDANT ANALYSES OF Ageratum conyzoides LEAF. Open Journal of Agricultural Science (ISSN: 2734-214X), 4(2), 17-28. https://doi.org/10.52417/ojas.v4i2.517